sonodynamic therapy using protoporphyrin ix conjugated to gold nanoparticles: an in vivo study on a colon tumor model

نویسندگان

ahmad shanei department of medical physics and medical engineering, school of medicine, isfahan university of medical sciences, isfahan, iran

ameneh sazgarnia research centre and department of medical physics, school of medicine, mashhad university of medical sciences, mashhad, iran

naser tayyebi meibodi skin research centre and department of pathology, emam reza hospital, mashhad university of medical sciences, mashhad, iran

hossein eshghi department of chemistry, school of science, ferdowsi university of mashhad, mashhad, iran

چکیده

objective(s) sonodynamic therapy is a physical treatment which utilizes ultrasound waves with an appropriate sensitizer such as protoporphyrin ix (ppix). the activation of sensitizer depends on cavitation, and therefore, high intensity ultrasound is an important necessity. beside, high intensity ultrasound can induce side effects on the healthy tissues which have surrounded tumor. the particles in a liquid decrease the ultrasonic intensity threshold needed for onset of cavitation. the non-radiative relaxation time of ppix in the presence of gold nanoparticles (gnp) is longer than the similar time without gnp. materials and methods this study was conducted on colon carcinoma tumor in balb/c mice. the tumors were induced by subcutaneous injection of ct26 cells. ultrasound irradiation were performed on tumors 24 hr after the injection of ppix into gnps. antitumor effects were estimated by measuring tumor relative volume, doubling time and time being five times of the tumors and by calculating the average survival time of tumor-bearing mice after treatment. results there is no inhibitory effect in control group. ultrasound irradiation alone showed a slight antitumor effect which was enhanced by ultrasound plus ppix (sdt). the synergistic inhibitory effect was significant when ultrasound plus ppix was conjugated to gnps. conclusion our experiments suggested a significant synergistic effect of ultrasound combined with au-ppix that reduced tumor relative volume and increased average animal survival fraction. this effect was obviously stronger than ultrasound alone and synergistic effect of ultrasound combined with ppix.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Sonodynamic Therapy Using Protoporphyrin IX Conjugated to Gold Nanoparticles: An In Vivo Study on a Colon Tumor Model

Objective(s) Sonodynamic therapy is a physical treatment which utilizes ultrasound waves with an appropriate sensitizer such as protoporphyrin IX (PpIX). The activation of sensitizer depends on cavitation, and therefore, high intensity ultrasound is an important necessity. Beside, high intensity ultrasound can induce side effects on the healthy tissues which have surrounded tumor. The particle...

متن کامل

Sonodynamic Therapy Using Protoporphyrin IX Conjugated to Gold Nanoparticles: An In Vivo Study on a Colon Tumor Model

OBJECTIVES Sonodynamic therapy is a physical treatment which utilizes ultrasound waves with an appropriate sensitizer such as protoporphyrin IX (PpIX). The activation of sensitizer depends on cavitation, and therefore, high intensity ultrasound is an important necessity. Beside, high intensity ultrasound can induce side effects on the healthy tissues which have surrounded tumor. The particles i...

متن کامل

Evaluation of Sonochemiluminescence in a Phantom in the Presence of Protoporphyrin IX Conjugated to Nanoparticles

Introduction When a liquid is irradiated with high-intensity and low-frequency ultrasound, acoustic cavitation occurs and there are some methods to determine and quantify this phenomenon. The existing methods for performing these experiments include sonochemiluminescence (SCL) and chemical dosimetric methods. The particles in a liquid decrease the ultrasonic intensity threshold needed for cavit...

متن کامل

A Study on the Photobleaching Effect of 5-ALA Conjugated Gold Nanoparticles in a CT26 Tumor Model During Photodynamic Therapy

Introduction During the process photodynamic therapy (PDT), bleaching of photosensitizer induced by irradiation and generation of reactive oxygen species (ROS) can provide some information concerning the efficiency of treatment. Since gold nanoparticles (GNPs) have been highlighted as efficient drug delivery systems, in this study, by utilizing GNPs conjugated with 5 aminolevulinic acid (5-ALA-...

متن کامل

a study on the photobleaching effect of 5-ala conjugated gold nanoparticles in a ct26 tumor model during photodynamic therapy

introduction during the process photodynamic therapy (pdt), bleaching of photosensitizer induced by irradiation and generation of reactive oxygen species (ros) can provide some information concerning the efficiency of treatment. since gold nanoparticles (gnps) have been highlighted as efficient drug delivery systems, in this study, by utilizing gnps conjugated with 5 aminolevulinic acid (5-ala-...

متن کامل

evaluation of sonochemiluminescence in a phantom in the presence of protoporphyrin ix conjugated to nanoparticles

introduction when a liquid is irradiated with high-intensity and low-frequency ultrasound, acoustic cavitation occurs and there are some methods to determine and quantify this phenomenon. the existing methods for performing these experiments include sonochemiluminescence (scl) and chemical dosimetric methods. the particles in a liquid decrease the ultrasonic intensity threshold needed for cavit...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۵، شماره ۲، صفحات ۷۵۹-۷۶۷

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023